annexin v fitc propidium iodide pi fluorescence probes  (Boster Bio)

Journal: Regenerative Therapy

Article Title: Targeting ER stress-mediated apoptosis by MSC-derived exosomes: A novel therapeutic strategy against pulmonary fibrosis

doi: 10.1016/j.reth.2025.10.004

Figure Lengend Snippet: BLM induced apoptosis in A549 cells. (A) Diagram of cell experiment. (B) Effects of BLM (25, 50, 100, 200 μg/ml) on the viability of A549 cells after 24 h. Cell viability was measured by CCK8 assay (n = 4, one-way ANOVA with Dunnett's test). (C) A549 cells were treated with BLM (100 μg/ml) for 24 h and 48 h. Percentage of total apoptotic cells were assessed with Annexin V/PI staining (n = 3, one-way ANOVA with Duncan's post hoc test). (D) A549 cells were treated with BLM (50, 100 μg/ml) for 24 h. The protein expression of Bcl-2 and Bax were measured via Western blotting, and the results were quantified using densitometry with ImageJ software (n = 3, one-way ANOVA with Duncan's post hoc test). Data are shown as the means ± SEM (∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 vs. the Cont group).

Article Snippet: Cells were stained with the Annexin V-FITC/propidium iodide (PI) fluorescence probes (MK1028, Boster, Wuhan, China), and the proportion of apoptotic cells was detected using flow cytometry.

Techniques: CCK-8 Assay, Staining, Expressing, Western Blot, Software

Journal: Regenerative Therapy

Article Title: Targeting ER stress-mediated apoptosis by MSC-derived exosomes: A novel therapeutic strategy against pulmonary fibrosis

doi: 10.1016/j.reth.2025.10.004

Figure Lengend Snippet: MSC-Ex reduced BLM-induced apoptosis in A549 cells. (A) A549 cells were treated with BLM (100 μg/ml) in the presence or absence of MSCs-Ex (100 μg/ml) for 24 h. (B) Cell viability was measured by CCK8 assay (n = 4, one-way ANOVA with Dunnett's test). (C) Percentage of total apoptotic cells was assessed with Annexin V/PI staining (n = 3, one-way ANOVA with Duncan's post hoc test). (D) and (E) The protein expression of Bcl-2 and Bax was measured via Western blotting, and the results were quantified using densitometry with ImageJ software (n = 3, one-way ANOVA with Duncan's post hoc test). Data are shown as the means ± SEM (∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 vs. the Cont group; # P < 0.05, ## P < 0.01 vs. the BLM group).

Article Snippet: Cells were stained with the Annexin V-FITC/propidium iodide (PI) fluorescence probes (MK1028, Boster, Wuhan, China), and the proportion of apoptotic cells was detected using flow cytometry.

Techniques: CCK-8 Assay, Staining, Expressing, Western Blot, Software

Journal: Regenerative Therapy

Article Title: Targeting ER stress-mediated apoptosis by MSC-derived exosomes: A novel therapeutic strategy against pulmonary fibrosis

doi: 10.1016/j.reth.2025.10.004

Figure Lengend Snippet: ER stress is involved in BLM-induced apoptosis in A549 cells. (A) Effects of TG (25, 50, 100, 200 μM) on the viability of A549 cells after 24 h. Cell viability was measured by CCK8 assay (n = 3, one-way ANOVA with Dunnett's test). (B) A549 cells were treated with TG (100 μM) for 24 h. Percentage of total apoptotic cells were assessed with Annexin V/PI staining (n = 3, one-way ANOVA with Duncan's post hoc test). (C) A549 cells were treated with TG (50 and 100 μM) for 24 h. The protein expression of Bcl-2 and Bax was measured via Western blotting, and the results were quantified using densitometry with ImageJ software (n = 3, one-way ANOVA with Duncan's post hoc test). Data are shown as the means ± SEM (∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 vs. the Cont group).

Article Snippet: Cells were stained with the Annexin V-FITC/propidium iodide (PI) fluorescence probes (MK1028, Boster, Wuhan, China), and the proportion of apoptotic cells was detected using flow cytometry.

Techniques: CCK-8 Assay, Staining, Expressing, Western Blot, Software

Journal: Regenerative Therapy

Article Title: Targeting ER stress-mediated apoptosis by MSC-derived exosomes: A novel therapeutic strategy against pulmonary fibrosis

doi: 10.1016/j.reth.2025.10.004

Figure Lengend Snippet: ER stress inhibition contributes to the anti-apoptotic effect of MSC-Ex. A549 cells were treated with BLM (100 μg/ml) in the presence or absence of TUDCA (100 μM) for 24 h. (A) The protein expression of BiP and CHOP was measured via Western blotting, and the results were quantified using densitometry with ImageJ software (n = 3, one-way ANOVA with Duncan's post hoc test). (B) Cell viability was measured by CCK8 assay (n = 4, one-way ANOVA with Dunnett's test). (C) Percentage of total apoptotic cells was assessed with Annexin V/PI staining (n = 3, one-way ANOVA with Duncan's post hoc test). (D) The protein expression of Bcl-2 and Bax was measured via Western blotting, and the results were quantified using densitometry with ImageJ software (n = 3, one-way ANOVA with Duncan's post hoc test). (E) The protein expression of BiP and CHOP in different groups was measured via Western blotting, and the results were quantified using densitometry with ImageJ software (n = 3, one-way ANOVA with Duncan's post hoc test). (F) Percentage of total apoptotic cells was assessed with Annexin V/PI staining (n = 3, one-way ANOVA with Duncan's post hoc test). Data are shown as the means ± SEM (∗ P < 0.05, ∗∗ P < 0.01, vs. the Cont group; # P < 0.05, ## P < 0.01 vs. the BLM/TG group).

Article Snippet: Cells were stained with the Annexin V-FITC/propidium iodide (PI) fluorescence probes (MK1028, Boster, Wuhan, China), and the proportion of apoptotic cells was detected using flow cytometry.

Techniques: Inhibition, Expressing, Western Blot, Software, CCK-8 Assay, Staining